Design and use of fluorescent fusion proteins in cell biology.
نویسنده
چکیده
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.
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ورودعنوان ژورنال:
- Current protocols in cell biology
دوره Chapter 21 شماره
صفحات -
تاریخ انتشار 2005